Carmo, Luciana Simao doRogero, Marcelo MacedoParedes-Gamero, Edgar JulianNogueira-Pedro, AmandaXavier, Jose Guilherme [UNESP]Cortez, MayaraBorges, Maria CarolinaFujii, Tatiane MiekoBorelli, PrimaveraFock, Ricardo Ambrosio2014-12-032014-12-032013-04-01Experimental Biology And Medicine. London: Royal Soc Medicine Press Ltd, v. 238, n. 4, p. 375-384, 2013.1535-3702http://hdl.handle.net/11449/111641It is well established that the excessive consumption of a high-fat diet (HFD) results in overweight, obesity and an increase in leptin concentrations, which triggers a chronic inflammatory condition that is associated with a high white blood cell count. Two-month-old male Wistar rats were fed a control (CON) diet or an HFD for 12 weeks. After this period, hemogram, myelogram and biochemical parameters were evaluated along with the cell cycle and the percentage of CD34(+) cells in the bone marrow as well as cell proliferation and differentiation assays and the production of stem cell factor, interleukin 3 (IL-3), granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF). The HFD animals exhibited leukocytosis and neutrophilia with increased C-reactive protein, leptin, cholesterol and triglyceride concentrations. In the HFD group, the bone marrow revealed myeloid hyperplasia, especially of the granulocytic compartment with a higher percentage of CD34(+) cells and a higher percentage of cells in the G2/S/M cell cycle phases. In addition, the HFD bone marrow cells had a higher capacity to proliferate and differentiate into granulocytic cells in an in vitro system and a higher capacity to produce IL-3 and G-CSF. These data led us to infer that the HFD induces leukocytosis and neutrophilia suggesting alterations in hematopoiesis system modulation.375-384enghigh-fat dietleukocytosisneutrophiliaIL-3G-CSFCD34 cellsArtigo10.1177/1535370213477976WOS:000321757900006Acesso restrito