Oliveira, Kassandra S. M. [UNESP]Di Bastiani, Mirela [UNESP]Cordeiro, Lucimara M. C.Costa, Mírian F. [UNESP]Toledo, Karina A. [UNESP]Iacomini, MarcelloBabosa, Aneli M.Dekker, Robert F. H.Nascimento, Valéria M. G. [UNESP]2015-12-072015-12-072015-11-20Carbohydrate Polymers, v. 133, p. 539-546, 2015.1879-1344http://hdl.handle.net/11449/131690The chemical composition and structural characterization of exopolysaccharides from the fungus Lasiodiplodia theobromae MMBJ are described, and the immunomodulatory activity of a purified β-glucan was evaluated. L. theobromae MMBJ produced three different β-glucans. One, fraction PEPS, was a branched (1→3)(1→6)-β-glucan and was insoluble in cold water. The other two, fractions SEPS-005R and SEPS-10E, were characterized as linear (1→6)-β-glucans with molar mass of 1.8×10(6)Da and 7.0×10(3)Da, respectively. From a total of 2.2g/L of EPS produced by L. theobromae through submerged fermentation, 1.5g/L (67%) was of the branched (1→3)(1→6)-β-glucan, while 25% (w/w) were linear (1→6)-β-glucans. Tests conducted with macrophages showed that the high molar mass (1→6)-β-glucan fraction (SEPS-005R) induced a pro-inflammatory response pattern.539-546engLasiodiplodanLinear (1→6)--glucan(1→3)(1→6)--GlucanExopolysaccharidesImmunomodulatory activity(1→6)- and (1→3)(1→6)-β-glucans from Lasiodiplodia theobromae MMBJ: structural characterization and pro-inflammatory activityArtigo10.1016/j.carbpol.2015.07.060Acesso restrito577256577430402026344312