Guimarães, Natalia S.S.Mello, Joyce C.Paiva, Juliana S.Bueno, Paula C.P. [UNESP]Berretta, Andresa A.Torquato, Ricardo J.Nantes, Iseli L.Rodrigues, Tiago2014-05-272014-05-272012-03-01Food and Chemical Toxicology, v. 50, n. 3-4, p. 1091-1097, 2012.0278-69151873-6351http://hdl.handle.net/11449/73200Baccharis dracunculifolia DC (Asteraceae) is the main botanical source used by honeybees to produce Brazilian green propolis whose hepatoprotective properties have been already described. In this work we investigated the protective effects of the glycolic extract of B. dracunculifolia (GEBd) against oxidative stress in isolated rat liver mitochondria (RLM). The GEBd was prepared by fractionated percolation using propylene glycol as solvent. The total phenols and flavonoids, which are substances with recognized antioxidant action, were quantified in GEBd and the phytochemical analysis was carried out by HPLC. GEBd exhibited significant scavenger activity towards DPPH radicals and superoxide anions in a concentration-dependent manner, and also a Fe 2+ chelating activity. GEBd decreased the basal H 2O 2 generation and the Fe 2+- or t-BuOOH-induced ROS production in isolated mitochondria. Lipid oxidation of mitochondrial membranes, protein thiol groups and GSH oxidation were also prevented by GEBd. This shows that B. dracunculifolia exhibit potent antioxidant activity protecting liver mitochondria against oxidative damage and such action probably contribute to the antioxidant and hepatoprotective effects of green propolis. © 2011 Elsevier Ltd.1091-1097engAntioxidantBaccharis dracunculifoliaFree radicalsHepatoprotectionMitochondriaOxidative stress1,1 diphenyl 2 picrylhydrazylantioxidantBaccharis dracunculifolia extractferrous ionflavonoidglutathionehydrogen peroxidephenolplant extractpropolispropylene glycolscavengersuperoxidethiol groupunclassified druganimal tissueantioxidant activityBaccharisbaccharis dracunculifoliacell protectionconcentration responsecontrolled studydown regulationdrug mechanismdrug potencyhigh performance liquid chromatographyiron chelationlipid oxidationliver mitochondrionliver protectionmalemitochondrial membranenonhumanoxidative stressphytochemistryratAnimalsAntioxidantsChromatography, High Pressure LiquidGlutathioneLipidsMaleMitochondria, LiverOxidation-ReductionOxidative StressPropolisRatsRats, WistarSpectrophotometry, UltravioletAsteraceaeRattusArtigo10.1016/j.fct.2011.11.014Acesso aberto2-s2.0-848564789272-s2.0-84856478927.pdf