Oliveira, Elisabete Schirato de [UNESP]Silva, Ketherson Rodrigues [UNESP]Fernando, Filipe Santos [UNESP]Mello Goncalves, Mariana Costa [UNESP]Fernandes, Camila Cesario [UNESP]Borzi, Mariana Monezi [UNESP]Santos, Romeu Moreira dos [UNESP]Feres Tamanini, Maria de Lourdes [UNESP]Silva Montassier, Maria de Fatima da [UNESP]Montassier, Helio Jose [UNESP]2014-12-032014-12-032013-11-01Journal Of Veterinary Diagnostic Investigation. Thousand Oaks: Sage Publications Inc, v. 25, n. 6, p. 720-726, 2013.1040-6387http://hdl.handle.net/11449/111466A competitive liquid-phase-blocking concanavalin A enzyme-linked immunosorbent assay (LPB-ConA-ELISA) was developed in the current study. The assay used ConA as a capture reagent, and the sera of specific pathogen-free chickens immunized with nonpurified Newcastle disease virus (NDV) suspension as detector antibodies, to detect and quantify specific antiviral antibodies in serum samples from free-ranging pigeons. The comparison between the LPB-ConA-ELISA and the hemagglutination inhibition (HI) test for the detection of antibodies in serum samples from 107 pigeons showed significant correlation between the assays (r = 0.875), a high sensitivity (100%), specificity (95.8%), accuracy (96.3%) for the ELISA, and good agreement ( = 0.83) between the 2 assays. The results of this study suggest that the LPB-ConA-ELISA could be a useful alternative to HI test in the serodiagnosis of NDV in pigeons, or other species of birds.720-726engImmunodiagnosticlectinsNewcastle disease viruspigeonsArtigo10.1177/1040638713503656WOS:000330221100005Acesso restrito0423131924105070