Martinhago, Ciro Dreschde Oliveira, Ricardo ManoelTomitao Canas, Maria do CarmoVagnini, Laura DinizAlcantara Oliveira, Joao BatistaPetersen, Claudia GuillherminoFranco Junior, Jose Gonçalves [UNESP]2014-05-202014-05-202006-12-01Prenatal Diagnosis. Chichester: John Wiley & Sons Ltd, v. 26, n. 13, p. 1219-1223, 2006.0197-3851http://hdl.handle.net/11449/12051Objective To assess the viability of the early diagnosis of fetal gender in material plasma before 7 weeks of pregnancy by real-time polymerase chain reaction (real-time PCR), starting at 5 weeks of pregnancy.Method peripheral blood was collected from pregnant women, starting at 5 weeks of gestation. After centrifugation, plasma was separated for fetal DNA extraction. DNA was analyzed by quantitative real-time PCR for two genomic regions, one on the Y chromosome (DYS-14) and the other shared by both sexes (beta-globin), by the TaqMan Minor Groove Binder (MGB) probe assay. The results of the examinations were compared to fetal gender determined after delivery.Results A total of 79 examinations of fetal DNA in maternal plasma were performed for 52 pregnant women. Accuracy according to gestational age was 92.6% (25 of 27 cases) at 5 weeks, and 95.6% (22 of 23 cases) at 6 weeks. These results also demonstrate that fetal DNA is present at low concentrations in maternal plasma at 5 weeks (8.5 genome equivalents (GE)/mL) and 6 weeks (34.1 GE/mL) of pregnancy.Conclusion Quantitative real-time PCR and TaqMan MGB probes specific for the detection of fetal gender in maternal plasma starting at 5 weeks of gestation have good sensitivity and excellent specificity. Copyright (c) 2006 John Wiley & Sons, Ltd.1219-1223engreal-time PCRTaqMan Minor Groove Binderfetal DNAmaternal plasmafetal sexingArtigo10.1002/pd.1592WOS:000243344300006Acesso restrito