Corradi Da Silva, Maria L. [UNESP]Exposti, Diego T.D. [UNESP]Vasconcelos, Ana F.D. [UNESP]Alexandre, Samara M.A. [UNESP]Silveira, Joana L.M.Ducatti, Diogo R.B.2014-05-272014-05-272013-10-02Carbohydrate Polymers, v. 98, n. 2, p. 1353-1359, 2013.0144-8617http://hdl.handle.net/11449/76775A water-soluble polysaccharide was extracted with alkali from the cell wall of Verticillium lecanii (also called Lecanicillium lecanii). After freezing and thawing, the water-soluble fraction was purified by gel filtration chromatography on Sepharose CL-6B and eluted as one peak by HPSEC/RID. Monosaccharide analysis showed galactose and glucose (1.1:1), with traces of mannose (<1%). The structural characteristics were determined by spectroscopic analysis, FT-IR and 1D and 2D 1H and 13C NMR, and methylation results. On the basis of the data obtained, the following structure of the polysaccharide (E3SIV fraction) was established: where n ≈ 22 and m ≈ 22. © 2013 Elsevier Ltd. All rights reserved.1353-1359engGlucogalactanLecanicillium lecaniiNMR spectroscopyVerticillium lecaniiArtigo10.1016/j.carbpol.2013.07.075WOS:000326313600014Acesso restrito2-s2.0-84884687189