Waltrick-Zambuzzi, MárciaTannure, Patricia NivoloniVieira, Thays Cristinedos SantosAntunes, Leonardo SantosRomano, Fábio LourençoZambuzzi, Willian Fernando [UNESP]Granjeiro, José MauroKüchler, Erika Calvano2018-12-112018-12-112015-11-01Brazilian Dental Journal, v. 26, n. 6, p. 561-565, 2015.1806-47600103-6440http://hdl.handle.net/11449/168292The aim of this study was to evaluate the association of the polymorphisms in TCN2 (rs1801198) gene and in MTRR (rs1801394) gene with nonsyndromic cleft lip and/or palate (NSCL/P) in a Brazilian population. Genomic DNA was extracted from buccal cells. The polymorphisms in TCN2 (rs1801198) and MTRR (rs1801394) genes were genotyped by carrying out real-time PCR and Taqman assay. Chi-square test was used to determine the association between genotype and allele frequencies with NSCL/P and NSCL/P subgroups (cleft lip only, cleft lip and palate, and cleft palate only). Eight hundred and sixty seven unrelated individuals (401 cases with NSCL/P and 466 individuals without cleft) were evaluated. Genotype distributions of TCN2 and MTRR polymorphisms were in Hardy-Weinberg equilibrium. The TCN2 polymorphic genotype GG was identified in 16.7% of the NSCL/P group and in 14.1% of the non-cleft group (p>0.05). Similarly, the frequency of MTRR genotype (GG) was similar in NSCL/P group (15.5%) and control group (17.8%) (p>0.05). Multivariate analysis showed an association between MTRR and the subgroup that the mother smoked during pregnancy (p=0.039). Our findings did not demonstrate an association between TCN2 polymorphisms and NSCL/P, however suggests an association between MTRR and NSCL/P etiology.561-565engCleft lip and/or palateFolic acidGenetic polymorphismsMTRR geneSubphenotypesTCN2 geneVitamin B12Artigo10.1590/0103-6440201300394S0103-64402015000600561Acesso aberto2-s2.0-84954071804S0103-64402015000600561.pdf