Guimaraes, Morgana Rodrigues [UNESP]Manzoli Leite, Fabio Renato [UNESP]Spolidório, Luis Carlos [UNESP]Kirkwood, Keith LoughRossa, Carlos [UNESP]2014-12-032014-12-032013-10-01Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 58, n. 10, p. 1309-1317, 2013.0003-9969http://hdl.handle.net/11449/112511Curcumin is the active compound in the extract of Curcuma longa rhizomes with anti-inflammatory properties mediated by inhibition of intracellular signalling. SOCS and MAP-Kinases are involved in the signalling events controlling the expression of IL-6, TNF-alpha and PGE(2), which have important roles on chronic inflammatory diseases. The aim was to assess if these pathways are involved in curcumin-mediated effects on LPS-induced expression of these cytokines in macrophages. RAW 264.7 murine macrophages were stimulated with Escherichia coli LPS in the presence and absence of non-cytotoxic concentrations of curcumin. Curcumin potently inhibited LPS-induced expression of IL-6, TNF-alpha and COX-2 mRNA and prevented LPS-induced inhibition of SOCS-1 and -3 expression and the inhibition of the activation of p38 MAPKinase by modulation of its nuclear translocation. In conclusion, curcumin potently inhibits expression of LPS-induced inflammatory cytokines in macrophages via mechanisms that involve modulation of expression and activity of SOCS-1 and SOCS-3 and of p38 MAPK. (C) 2013 Elsevier Ltd. All rights reserved.1309-1317engCurcuminInnate immunityCell signallingCytokinesNF-kappa BMAPKSOCSArtigo10.1016/j.archoralbio.2013.07.005WOS:000325236600006Acesso restrito2640929291808415