Rocha, Claudia Quintino da [UNESP]Queiroz, Emerson FerreiraMeira, Cassio SantanaMagalhaes Moreira, Diogo RodrigoPereira Soares, Milena BotelhoMarcourt, LaurenceVilegas, Wagner [UNESP]Wolfender, Jean-Luc2015-03-182015-03-182014-06-01Journal Of Natural Products. Washington: Amer Chemical Soc, v. 77, n. 6, p. 1345-1350, 2014.0163-3864http://hdl.handle.net/11449/116837The nonpolar fraction of an aqueous ethanol extract of the roots of Arrabidaea brachypoda, a Brazilian medicinal plant, demonstrated significant in vitro activity against Trypanosoma cruzi, the parasite responsible for Chagas disease. Targeted isolation of the active constituents led to the isolation of three new dimeric flavonoids (1-3), and their structures were elucidated using UV, NMR, and HRMS analysis, as well as by chemical derivatization. The anti-T. cruzi activity and cytotoxicity toward mammalian cells were determined for these substances. Compound 1 exhibited no activity toward T. cruzi, while flavonoids 2 and 3 exhibited selective activity against these trypomastigotes. Compounds 2 and 3 inhibited the parasite invasion process and its intracellular development in host cells with similar potencies to benznidazole. In addition, compound 2 reduced the blood parasitemia of T. cruzi-infected mice. This study has revealed that these two dimeric flavonoids represent potential anti-T. cruzi lead compounds for further drug development.1345-1350engArtigo10.1021/np401060jWOS:000340222700014Acesso restrito79278772243268370000-0003-3032-2556