Costa, GessycaFernandes, AndréiaSantos, ThaísBrito, LaraRodrigues, LaísValadares, MarizeFelzenszwalb, IsraelFerraz, Elisa [UNESP]Morais Leme, Daniela [UNESP]Oliveira, Gisele [UNESP]2022-04-282022-04-282022-01-01Journal of Toxicology and Environmental Health - Part A: Current Issues.1087-26201528-7394http://hdl.handle.net/11449/223540Resistance to glyphosate herbicide has initiated usage of combined application of herbicides as a weed control measure. Imazethapyr-based herbicides associated with glyphosate herbicide seem to be an alternative to suppress weed resistance. The aim of this study was to examine the adverse effects of Glyphosate Atanor 48® (ATN) and Imazethapyr Plus Nortox® (IMZT) formulations in both single forms and mixtures using HepG2 cells and zebrafish early-life stages models. Data demonstrated cytotoxicity due to exposure to ATN, IMZT for both models, as follows: (1) ATN (0.5 mg/L), IMZT (5 mg/L), and M3 (0.05 mg/L ATN + 5 mg/L IMZT) increased cytotoxicity by disturbing the mitochondrial activity of HepG2 cells 24 hr after exposure; (2) ATN and IMZT (5 mg/L), and M3 (0.05 mg/L ATN + 5 mg/L IMZT) also decreased the integrity of the membrane of HepG2 cells after 24 hr incubation; (3) only ATN and IMZT (5 mg/L) in their single forms diminished the mitochondrial potential of zebrafish; (4) ATN (single form) at 0.5 mg/L induced apoptosis in zebrafish larvae. In conclusion, these herbicides in their single forms appeared to produce greater cytotoxicity to HepG2 cells and zebrafish compared to the herbicide mixtures.engcytotoxicityFormulationsHepG2mixtureszebrafishArtigo10.1080/15287394.2022.20362812-s2.0-85125470190