Barbieri, C. M O [UNESP]Costa, Paulo Inácio da [UNESP]2014-05-272014-05-272004-12-01Revista de Ciencias Farmaceuticas, v. 25, n. 2, p. 129-134, 2004.0101-3793http://hdl.handle.net/11449/68055Human platelet-derived growth factor (PDGF) was purified from lysates of clinically outdated human platelets by ionic exchange chromatography in CM-Sepharose. The eluated fraction was submitted to the Immunoblot/Slot Blot assay using anti-PDGF-AA and anti-PDGF-BB polyclonal antibodies and was evaluated as to its biological activity through the test of [H 3]-thymidine incorporation in NIH/3T3 cell line fibroblasts in culture. The Immunoblot/Slot Blot assay using anti-PDGF-AA and anti-PDGF-BB antibodies proved the presence of the PDGF in chromatographic cationic fraction. The comparison of biological activities between fiblobrast stimulation assay using recombinant PDGF-AB and partially purified PDGF was demonstrated in 165.796 and 157.567 cpm, respectively. This result, proved the potent mitogenic effect of partially purified PDGF and consequently their evidence about the wound healing activity.129-134porPDGFPlateletsWound healingcationplatelet derived growth factorplatelet derived growth factor AAplatelet derived growth factor ABplatelet derived growth factor BBpolyclonal antibodyrecombinant platelet derived growth factorsepharosethymidinetritiumwound healing promoting agentanimal cellcell lysatecell stimulationcell strain 3T3controlled studydrug activitydrug costdrug potencydrug purificationdrug screeningfibroblast cultureimmunoblottingion exchange chromatographymethodologymitogenesismousenonhumanslot blot hybridizationthrombocytewound healingArtigoAcesso restrito2-s2.0-2744444111667202237159173810000-0002-3350-8308