Espindola, Foued S.Banzi, Silmara R.Calabria, Luciana K.Custódio, Rodrigo J.Oliveira, Ricardo A.Procópio, Leandro D.Lima, Andreia B. P.Cunha-Junior, Jair P.Coelho, Milton V.Guedes, Iêda M. L.Pellizzon, Cláudia H. [UNESP]Larson, Roy E.Espreafico, Enilza M.2014-05-272014-05-272008-08-01Cell and Tissue Research, v. 333, n. 2, p. 263-279, 2008.0302-766Xhttp://hdl.handle.net/11449/70500Myosin-Va is a Ca 2+/calmodulin-regulated unconventional myosin involved in the transport of vesicles, membranous organelles, and macromolecular complexes composed of proteins and mRNA. The cellular localization of myosin-Va has been described in great detail in several vertebrate cell types, including neurons, melanocytes, lymphocytes, auditory tissues, and a number of cultured cells. Here, we provide an immunohistochemical view of the tissue distribution of myosin-Va in the major endocrine organs. Myosin-Va is highly expressed in the pineal and pituitary glands and in specific cell populations of other endocrine glands, especially the parafollicular cells of the thyroid, the principal cells of the parathyroid, the islets of Langerhans of the pancreas, the chromaffin cells of the adrenal medulla, and a subpopulation of interstitial testicular cells. Weak to moderate staining has been detected in steroidogenic cells of the adrenal cortex, ovary, and Leydig cells. Myosin-Va has also been localized to non-endocrine cells, such as the germ cells of the seminiferous epithelium and maturing oocytes and in the intercalated ducts of the exocrine pancreas. These data provide the first systematic description of myosin-Va localization in the major endocrine organs of rat. © 2008 Springer-Verlag.263-279engEndocrine glandsGerm cellsImmunohistochemistryMyosin-VaNeuroendocrine cellsRat (Wistar)messenger RNAmyosin Vaprotein kinase (calcium,calmodulin)adrenal cortexanimal cellanimal tissuecell specificitycell subpopulationcell typecellular distributionchromaffin cellcontrolled studyendocrine cellhypophysisimmunohistochemistryLeydig celllymphocytemacromoleculemelanocytemembrane vesiclenerve cellnonhumanoocyte maturationovary cellpancreas isletparathyroid cellpineal bodypriority journalprotein expressionprotein localizationratseminiferous tubule epitheliumsteroidogenesistestis cellthyroid follicle celltissue distributionAdrenal GlandsAnimalsEndocrine GlandsImmunoblottingIslets of LangerhansMyosin Heavy ChainsMyosin Type VParathyroid GlandsPineal GlandPituitary GlandRatsThyroid GlandTissue DistributionRattusVertebrataArtigo10.1007/s00441-008-0630-8Acesso restrito2-s2.0-4814910271900193937798010690000-0002-4494-4180