Nunes, CRMLima, LGFManoel, C. S.Pereira, R. N.Nakano, M. M.Garcia, José Fernando [UNESP]2014-02-262014-05-202014-02-262014-05-202003-05-01Experimental Parasitology. San Diego: Academic Press Inc. Elsevier B.V., v. 104, n. 1-2, p. 67-69, 2003.0014-4894http://hdl.handle.net/11449/14718The taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T saginata identification in human fecal samples. (C) 2003 Elsevier B.V. (USA). All rights reserved.67-69engArtigo10.1016/S0014-4894(03)00111-5WOS:000185132300010Acesso restrito9991374083045897