Matos, Adaias OliveiraRangel, Elidiane Cipriano [UNESP]Barão, Valentim Adelino RicardoGregory, Richard Lee2023-07-292023-07-292023-01-01Dental Materials Journal, v. 42, n. 2, p. 228-235, 2023.1881-13610287-4547http://hdl.handle.net/11449/249808Because nicotine upregulates the growth of most oral bacteria, this in vitro study investigated the antimicrobial effect of chlorhexidine-doped thin film on commercially pure titanium against Fusobacterium nucleatum (F. nucleatum) biofilm supplemented with different concentrations of nicotine (0, 1, and 2 mg/mL). Biofilms were formed on a chlorhexidine-doped thin film on commercially-pure-titanium discs and compared to the control groups. Biofilm viability, total biofilm growth using a spectrophotometer, extracellular polysaccharide content, and pH variations were assessed as dependent variables. Data were submitted to ANOVA and Tukey honest significant difference tests (α=0.05). F. nucleatum biofilm growth was inhibited when exposed to chlorhexidine-doped thin film (p<0.05). Biofilm supplemented with nicotine did not impact the synthesis of EPS on the same type of treatment (p>0.05). The pH values were significantly increased with the increase of nicotine concentration (p<0.05). Chlorhexidine-doped thin film was effective in reducing F. nucleatum biofilm supplemented with nicotine.228-235engBiofilmChlorhexidineFusobacterium nucleatumNicotineTitaniumArtigo10.4012/dmj.2022-1682-s2.0-85151313218