Chiodi Borges, Aline [UNESP]Castaldelli Nishime, Thalita Mayumi [UNESP]Kostov, Konstantin Georgiev [UNESP]de Morais Gouvêa Lima, Gabriela [UNESP]Vidal Lacerda Gontijo, Aline [UNESP]Nóbrega Martins Marchesotti de Carvalho, Juliana [UNESP]Yzumi Honda, Roberto [UNESP]Koga-Ito, Cristiane Yumi [UNESP]2018-12-112018-12-112017-12-01Clinical Plasma Medicine, v. 7-8, p. 9-15.2212-8166http://hdl.handle.net/11449/179376The occurrence of Candida-related infections depends on the interplay between fungal virulence factors and host's immune system. For this reason, interfering with fungal virulence is a promising approach to prevent or treat such diseases. The goal of this study was to analyze the effects of cold atmospheric pressure plasma jet operated with Helium (He-CAPPJ) on C. albicans virulence traits and biofilms. The study also aimed to evaluate the cytotoxicity and genotoxicity of He-CAPPJ to Vero cells. The effective operational parameters of He-CAPPJ against C. albicans SC 5314 were determined. Sub-inhibitory times of exposure were used to evaluate the effect of He-CAPPJ on morphogenesis (yeast-hyphae transition), adherence to epithelial cells, and exoenzymes production. Cytotoxicity to Vero cells was also assessed by MTT assay and genotoxicity was investigated by micronucleus test. A promising effect of He-CAPPJ on morphogenesis was observed, with a reduction of almost 40 times in the filamentation rate compared to the non-exposed group. He-CAPPJ was able to reduce C. albicans adherence and to decrease fungal biofilm viability. No effect on exoenzymes production was observed. In conclusion, He-CAPPJ was able to attenuate fungal adherence and morphogenesis with low cytotoxicity after short treatment times. Periods of exposure effective against biofilms were cytotoxic to Vero cells. He-CAPPJ did not show genotoxic effect. Therefore, He-CAPPJ has potential to be applied as an adjuvant in the treatment of C. albicans superficial infections.9-15engAntifungalBiofilmsCandida albicansCytotoxicityPlasmaVirulenceArtigo10.1016/j.cpme.2017.06.002Acesso aberto2-s2.0-850346520052-s2.0-85034652005.pdf194650980100045065435631614034210000-0002-2416-2173