Pepato, Maria Teresa [UNESP]Baviera, Amanda Martins [UNESP]Vendramini, Regina Célia [UNESP]Brunetti, Iguatemy Lourenço [UNESP]2014-05-272014-05-272004-06-08BMC Complementary and Alternative Medicine, v. 4.1472-6882http://hdl.handle.net/11449/67764Background: Previous experiments have shown that a decoction of Bauhinia forficata leaves reduces the changes in carbohydrate and protein metabolism that occur in rats with streptozotocin-induced diabetes. In the present investigation, the serum activities of enzymes known to be reliable toxicity markers were monitored in normal and streptozotocin-diabetic rats to discover whether the use of B. forficata decoction has toxic effects on liver, muscle or pancreas tissue or on renal microcirculation. Methods: An experimental group of normal and streptozotocin-diabetic rats received an aqueous decoction of fresh B. forficata leaves (150 g/L) by mouth for 33 days while a control group of normal and diabetic rats received water for the same length of time. The serum activity of the toxicity markers lactate dehydrogenase, creatine kinase, amylase, angiotensin-converting enzyme and bilirubin were assayed before receiving B. forficata decoction and on day 19 and 33 of treatment. Results: The toxicity markers in normal and diabetic rats were not altered by the diabetes itself nor by treatment with decoction. Whether or not they received B. forficata decoction the normal rats showed a significant increase in serum amylase activity during the experimental period while there was a tendency for the diabetic rats, both treated and untreated with decoction, to have lower serum amylase activities than the normal rats. Conclusions: Administration of an aqueous decoction of B. forficata is a potential treatment for diabetes and does not produce toxic effects measurable with the enzyme markers used in our study. © 2004 Pepato et al; licensee BioMed Central Ltd.engAmylaseAngiotensin-converting enzymeBauhinia forficataBilirubinCreatine kinaseExtended treatmentLactate dehydrogenaseSerum enzymesStreptozotocin-diabetic ratsamylaseBauhinia forticata extractbilirubincreatine kinasedipeptidyl carboxypeptidaselactate dehydrogenaseplant extractstreptozocinunclassified drugwateramylase blood levelanimal experimentanimal modelanimal tissueaqueous solutionBauhiniabilirubin blood levelcontrolled studycreatine kinase blood leveldiabetes mellitusenzyme activityenzyme assayenzyme blood levelkidney circulationlactate dehydrogenase blood levelliver toxicitymalemicrocirculationmuscle diseasenephrotoxicitynonhumanpancreas diseaseplant leafratstreptozocin diabetesanimalchemically induced disorderexperimental diabetes mellitusmetabolismphytotherapytoxicity testingWistar ratAmylasesAnimalsCreatine KinaseDiabetes Mellitus, ExperimentalL-Lactate DehydrogenaseMalePeptidyl-Dipeptidase APhytotherapyPlant LeavesRatsRats, WistarStreptozocinToxicity Tests, ChronicArtigo10.1186/1472-6882-4-7Acesso aberto2-s2.0-104442391902-s2.0-10444239190.pdf37364750251877507641979287850489