de Siqueira-Silva, Diógenes Henrique [UNESP]dos Santos Silva, Amanda Pereira [UNESP]da Silva Costa, Raphael [UNESP]Senhorini, José AugustoNinhaus-Silveira, Alexandre [UNESP]Veríssimo-Silveira, Rosicleire [UNESP]2019-10-062019-10-062019-01-01Fish Physiology and Biochemistry.1573-51680920-1742http://hdl.handle.net/11449/188991We aimed to develop a simplified protocol for transplantation of Brycon orbignyanus spermatogonial stem cells (SSCs) into Astyanax altiparanae testes. Brycon orbignyanus testes were enzymatically digested and SSC purified by a discontinuous density gradient. Endogenous spermatogenesis was suppressed in A. altiparanae using busulfan or by incubation at 35 °C water, and SSCs from B. orbignyanus labeled with PKH26 were injected into their testes via the urogenital papilla. Twenty-two hours post-transplantation, labeled spermatogonia were observed in A. altiparanae tubular lumen. After 7 days, spermatogonia proliferated in the epithelium, and 21 days post-transplantation, sperm was observed in the lumen. Of surviving host fish, nearly 67% of those treated with busulfan and 85% of those held in warm water showed labeled cells in host germinal epithelium. The present study standardized, by a simple and accessible method, germ cell transplantation between sexually mature Characiformes fish species. This is the first report of xenogenic SSC transplantation in this fish order.engFish chimeraGerm cell technologySpermatogonial proliferationSurrogate productionArtigo10.1007/s10695-019-00631-8Acesso aberto2-s2.0-8506445527329260539849144770000-0002-8298-5004