Baeza-Fonte, Alen NGarcés-Lobo, IdeniaLuaces-Alberto, Markel DGonçalves, Luís MoreiraSotomayor, Maria D P T [UNESP]Valdés-González, Arístides C2018-12-112018-12-112018-02-01Journal of Chromatographic Science, v. 56, n. 2, p. 187-193, 2018.0021-9665http://hdl.handle.net/11449/175849Molecularly imprinted polymers (MIPs) were synthesized for the determination of cephalosporins (cephazolin, ceftazidime, cefotaxime, ceftriaxone, cefepime and cephalexin) by ultra-high performance liquid chromatography with diode-array detection (UHPLC-DAD). After optimization, MIPs were synthesized using cephazolin as the template, methacrylic acid as the functional monomer, triethylenglycol dimethacrylate as the crosslinker, acetonitrile/dimethylsulfoxide as porogens and benzoyl peroxide as the radical initiator. Not only this is a novel route of MIP synthesis for cephalosporins, but also this choice of analytes is unique. Chromatographic separation was performed in a C 8 column using a binary gradient with trifluoroacetic acid 0.1% in water and acetonitrile. Linearity was assessed up to 100 μg mL -1 and linear correlation coefficients (r) were all higher than 0.99, limits of detection were within the range of 3-12 ng mL -1, and recoveries from 86 to 102% were obtained for concentrations between 0.05 and 1.0 μg mL -1.187-193engArtigo10.1093/chromsci/bmx099Acesso aberto2-s2.0-850416608922-s2.0-85041660892.pdf