Lima, Paula M. [UNESP]Neves, Renato de C. F. [UNESP]dos Santos, Felipe A. [UNESP]Perez, Carlos A.da Silva, Marcelo O. A.Arruda, Marco A. Z.de Castro, Gustavo R. [UNESP]Padilha, Pedro M. [UNESP]2014-05-202014-05-202010-08-15Talanta. Amsterdam: Elsevier B.V., v. 82, n. 3, p. 1052-1056, 2010.0039-9140http://hdl.handle.net/11449/19127An investigation was made into calcium, iron and zinc in protein spots in samples of Nile tilapia (Oreochromis niloticus) liver tissue obtained after protein separation by two-dimensional polyacrylamide gel electrophoresis (20-PAGE) and subsequent qualitative and quantitative evaluation by synchrotron radiation X-ray fluorescence (SRXRF) and Flame Atomic Absorption Spectrometry (FAAS). An analysis of the fluorescence spectra indicated the presence of calcium, iron and zinc in 12, 6 and 8 liver protein spots, respectively. The metal ions found were distributed mainly in proteins with a molar mass of less than 40.00 kDa and more than 12.00 kDa, with pI in the range of 4.70-9.40. The only exception was a spot presenting protein with a molar mass of 10.10 kDa. In addition to calcium, iron and zinc, sulfur and phosphorus - which are non-metals that may be part of the protein structure, were also detected. After microwave-assisted acid mineralization of the proteins spots, a FAAS estimation of the concentration of calcium, iron and zinc ions bound to these proteins indicated a range of 1.08-5.80 mg g(-1), 2.02-8.03 mg g(-1) and 1.60-8.55 mg g(-1), respectively. (C) 2010 Published by Elsevier B.V.1052-1056engMetallomicsProtein spots2D-PAGESRXRFFAASArtigo10.1016/j.talanta.2010.06.023WOS:000281177100029Acesso restrito69814486374563910000-0003-4179-0574