Fontana, R.Mendes, M. A.de Souza, B. M.Konno, K.Cesar, LMMMalaspina, O.Palma, Mario Sergio [UNESP]2014-05-202014-05-202004-06-01Peptides. New York: Elsevier B.V., v. 25, n. 6, p. 919-928, 2004.0196-9781http://hdl.handle.net/11449/19600Four antimicrobial peptides were purified from Royal Jelly of honeybees, by using reverse phase-HPLC and sequenced by using Q-Tof-MS/MS: PFKLSLHL-NH2 (Jelleine-I), TPFKLSLHL-NH2 (Jelleine-II), EPFKLSLHL-NH2 (Jelleine-III), and TPFKLSLH-NH2 (Jelleine-IV). The peptides were synthesized on-solid phase, purified and submitted to different biological assays: antimicrobial activity, mast cell degranulating activity and hemolysis. The Jelleines-I-III presented exclusively antimicrobial activities against yeast, Gram+ and Gram- bacteria; meanwhile, Jelleine-IV was not active in none of the assays performed. These peptides do not present any similarity with the other antimicrobial peptides from the honeybees; they are produced constitutively by the workers and secreted into Royal Jelly. (C) 2004 Elsevier B.V. All rights reserved.919-928engroyal jellyAfricanized honeybeesApis melliferamass spectrometryantimicrobial peptidesArtigo10.1016/j.peptides.2004.03.016WOS:000222395900003Acesso restrito75385560855058192901888624506535