Berretta, Andresa AparecidaNascimento, Andresa PiacezziPires Bueno, Paula Carolina [UNESP]Lima Leite Vaz, Mirela Mara de OliveiraMarchetti, Juliana Maldonado2014-05-202014-05-202012-01-01International Journal of Biological Sciences. Lake Haven: Ivyspring Int Publ, v. 8, n. 4, p. 512-521, 2012.1449-2288http://hdl.handle.net/11449/42430The aim of this study was to develop a formulation, containing the propolis standardized extract (EPP-AF (R)), which can assist in the healing of skin lesions. To achieve this objective the antimicrobial activity and chemical composition of the propolis extract was determined. The final product was subjected to in vitro and in vivo pre-clinical evaluation. The broth macrodi-lution method was used to determine the antimicrobial activity of the extracts and formulations against the microorganisms most commonly found in burns, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis. Wistar rats with puncture wounded skin were used to evaluate the wound healing properties of propolis. The results of chemical and biological characterization demonstrated the batch-to-batch reproducibility of the standardized extract which is an unprecedented result. The antimicrobial and wound healing activity of the pharmaceutical studied showed the best results when samples contain 3.6% propolis, suggesting that this is the most promising composition.512-521engpropolisEPP-AF (R)antimicrobial activitywound healingpharmaceuticsreproducibilityArtigo10.7150/ijbs.3641WOS:000303805500008Acesso abertoWOS000303805500008.pdf