Bonfilio, RudyTeixeira Tarley, Cesar RicardoPereira, Gislaine RibeiroSalgado, Hérida Regina Nunes [UNESP]de Araujo, Magali Benjamim2014-05-202014-05-202009-11-15Talanta. Amsterdam: Elsevier B.V., v. 80, n. 1, p. 236-241, 2009.0039-9140http://hdl.handle.net/11449/7835This paper describes the optimization and validation of an analytical methodology for the determination of losartan potassium in capsules by HPLC using 2(5-1) fractional factorial and Doehlert designs. This multivariate approach allows a considerable improvement in chromatographic performance using fewer experiments, without additional cost for columns or other equipment. The HPLC method utilized potassium phosphate buffer (pH 6.2: 58 mmol L(-1))-acetonitrile (65:35, v/v) as the mobile phase, pumped at a flow rate of 1.0 mL min(-1). An octylsilane column (100 mm x 4.6 mm W., 5 mu m) maintained at 35 degrees C was used as the stationary phase. UV detection was performed at 254 nm. The method was validated according to the ICH guidelines, showing accuracy, precision (intra-day relative standard deviation (R.S.D.) and inter-day R.S.D values <2.0%). selectivity, robustness and linearity (r=0.9998) over a concentration range from 30 to 70 mg L(-1) of losartan potassium. The limits of detection and quantification were 0.114 and 0.420 mg L(-1), respectively. The validated method may be used to quantify losartan potassium in capsules and to determine the stability of this drug. (C) 2009 Elsevier B.V. All rights reserved.236-241engDoehlert designFractional factorial designLosartan potassiumReversed-phase chromatographyValidationTrabalho apresentado em evento10.1016/j.talanta.2009.06.060WOS:000271055700036Acesso aberto