Domingues, Marco M.Bianconi, M. LuciaBarbosa, Leandro R.S.Santiago, Patrícia S. [UNESP]Tabak, MarcelCastanho, Miguel A.R.B.Itri, RosangelaSantos, Nuno. C.2014-05-272014-05-272013-08-27Biochimica et Biophysica Acta - Biomembranes, v. 1828, n. 11, p. 2419-2427, 2013.0005-27361879-2642http://hdl.handle.net/11449/76336rBPI21 belongs to the antimicrobial peptide and protein (AMP) family. It has high affinity for lipopolysaccharide (LPS), acting mainly against Gram-negative bacteria. This work intends to elucidate the mechanism of action of rBPI21 at the membrane level. Using isothermal titration calorimetry, we observed that rBPI21 interaction occurs only with negatively charged membranes (mimicking bacterial membranes) and is entropically driven. Differential scanning calorimetry shows that membrane interaction with rBPI21 is followed by an increase of rigidity on negatively charged membrane, which is corroborated by small angle X-ray scattering (SAXS). Additionally, SAXS data reveal that rBPI21 promotes the multilamellarization of negatively charged membranes. The results support the proposed model for rBPI21 action: first it may interact with LPS at the bacterial surface. This entropic interaction could cause the release of ions that maintain the packed structure of LPS, ensuring peptide penetration. Then, rBPI21 may interact with the negatively charged leaflets of the outer and inner membranes, promoting the interaction between the two bacterial membranes, ultimately leading to cell death. © 2013 Elsevier B.V.2419-2427engAMPLipopolysaccharideMembrane bindingMicrocalorimetryrBPI21SAXSlipopolysacchariderecombinant bactericidal permeability increasing proteinapoptosisbacterial membranecell structuredifferential scanning calorimetryGram negative bacteriumisothermal titration calorimetrylamellar bodymembrane bindingmembrane leafletmembrane permeabilitymolecular interactionnonhumanphysical chemistrypriority journalprotein interactionprotein structurerigid multilamellar structurerigidityX ray crystallographyArtigo10.1016/j.bbamem.2013.06.009WOS:000326143200008Acesso restrito2-s2.0-8488260778867053670106620870000-0002-6205-9441