Trevisan, Henrique Celso [UNESP]Medeiros, João Batista de [UNESP]Lisboa, Helen Cristina Fávero [UNESP]2014-05-202014-05-202006-07-01Química Nova. Sociedade Brasileira de Química, v. 29, n. 4, p. 865-867, 2006.0100-4042http://hdl.handle.net/11449/25349A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4°C.865-867porporcine liver esterasePLEbiocatalysisArtigo10.1590/S0100-40422006000400040S0100-40422006000400040Acesso abertoS0100-40422006000400040.pdf