Mota, Adolfo JoseBack Brito, Graziella Nuernberg [UNESP]Nobrega, Francisco G. [UNESP]2014-05-202014-05-202012-01-01Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 35, n. 1, p. 122-125, 2012.1415-4757http://hdl.handle.net/11449/22712Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was validated by DNA sequencing of the polymorphic region used in a real-time PCR assay driven by species-specific probes targeted to the fungal ITS 1 region. This assay provides a new tool for pathogen identification and for epidemiological, drug resistance and virulence studies of these organisms.122-125engCandida palmioleophilaDebaryomyces hanseniidifferential identificationPichia guilliermondiireal-time PCRArtigo10.1590/S1415-47572011005000059S1415-47572012000100017WOS:000301305200017Acesso abertoS1415-47572012000100017.pdf