Santos, L. C.Dachtler, M.Andrade, F. D. P.Albert, K.Vilegas, Wagner [UNESP]2014-05-272014-05-272000-12-01Fresenius' Journal of Analytical Chemistry, v. 368, n. 5, p. 540-542, 2000.0937-0633http://hdl.handle.net/11449/66291The on-line separation and identification of two important taxonomic markers for plant species of the Paepalanthus genus, the flavonoids 6-methoxykaempferol-3-O-β-D-glucopyranoside and 6-methoxykaempferol-3-O-β-D-6″(p-coumaroyl)glucopyranoside, has been performed with an HPLC-NMR coupling using C30 phase. 1D spectra have been recorded in the stopped-flow mode for the two predominant chromatographic peaks. This is the first application of HPLC-NMR coupling using C30 phase to a taxonomic problem. The technique drastically reduces the required amount of sampling for structure determination. © Springer-Verlag 2000.540-542engflavonoidchemistryclassificationequipment designhigh performance liquid chromatographyisolation and purificationmedicinal plantmethodologynuclear magnetic resonance spectroscopyonline systemstandardChromatography, High Pressure LiquidEquipment DesignFlavonoidsMagnetic Resonance SpectroscopyOnline SystemsPlants, MedicinalArtigo10.1007/s002160000534Acesso restrito2-s2.0-003433242479278772243268370000-0003-3032-2556