Verdichio-Moraes, Camila Fernanda [UNESP]Toralles-Pereira, Cecilia [UNESP]Grotto, Rejane Maria Tommasini [UNESP]Silva, Giovanni Faria [UNESP]Pardini, Maria Ines de Moura Campos [UNESP]2014-05-202014-05-202009-04-01Journal of Medical Virology. Hoboken: Wiley-liss, v. 81, n. 4, p. 757-759, 2009.0146-6615http://hdl.handle.net/11449/11465Studies have suggested that hepatitis C virus (HCV) may infect not only hepatocytes but may also be carried by platelets. Platelets express more than 20 polymorphic antigenic determinants on their surface, which are called human platelet antigens (HPA), To determine the allele frequency of the HPA-1 to -5 in patients infected with HCV, blood samples were collected from 257 blood donors for the control group and from 191 patients infected with HCV. DNA was isolated and amplified for genes HPA-1 to -4 using PCR Sequence Specific Primers (PCR-SSP) and HPA-5 using PCR-Restriction Fragment Length Polymorphism (PCR-RFLP). The allelic and genotypic frequency of HPA-5a in patients infected with HCV was found to be significantly lower(P < 0.05) than in the controls, and HPA-5b from patients infected with HCV was significantly higher (P < 0.05) than in controls. The increase in HPA5b allelic frequency in HCV infection may indicate a possible association between HCV infection and HPAs. J. Med. Virol. 81:757-759, 2009. (C) 2009 Wiley-Liss, Inc.757-759engallelic frequencieshepatitis C virushuman platelet antigensplateletsArtigo10.1002/jmv.21411WOS:000263765900026Acesso restrito6322604200510676461958833458208477884485643265850000-0002-4035-9486