Semliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals production

Núñez, Eutimio Gustavo Fernández [UNESP]Jorge, Soraia Attie CalilAstray, Renato Mancinide Rezende, Alexandre Gonçalvesda Costa, Bruno Labate ValeMonteiro, Daniella Cristina VentiniPereira, Carlos AugustoTonso, Aldo2022-04-292022-04-292013-09-01Brazilian Archives of Biology and Technology, v. 56, n. 5, p. 859-866, 2013.1516-89131678-4324http://hdl.handle.net/11449/227294The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.859-866engBHK-21Large-scale bioprocessesMammalian cellsRecombinant proteinsSFVTransient expressionSemliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals productionArtigo10.1590/S1516-891320130005000182-s2.0-84887180324