Fonseca-Santos, Bruno [UNESP]Gremião, Maria Palmira Daflon [UNESP]Chorilli, Marlus [UNESP]2018-12-112018-12-112017-11-01Arabian Journal of Chemistry, v. 10, n. 7, p. 1029-1037, 2017.1878-5352http://hdl.handle.net/11449/172543A simple, rapid, and sensitive analytical procedure has been developed and validated for the in vitro measurement of curcumin in samples from mucosae retention studies. Curcumin was analyzed by HPLC using a C18 column with UV detection at 425 nm. The mobile phase was acetonitrile and water (50:50 v/v) acidified with 2% acetic acid at a flow rate of 1.2 mL min−1. The curve range was linear for the receptor solution concentration range 0.5–75 μg mL−1. The specificity showed no interference with the biological matrix and excipients of the acceptor media. Intra and inter-day accuracy, and precision values were lower than 5% and were not statically different (P < 0.05). Recoveries ranged from 99.7% to 108%. The limits of detection and quantitation were 11.61 and 500 ng mL−1, respectively. The method is adequate to assay curcumin from esophageal porcine samples, enabling the determination of penetration profiles for in situ gelling curcumin-loaded liquid crystals by in vitro studies, and fulfilled the requirements for reliability and feasibility for application to the quantitative analysis of curcumin in porcine esophageal mucosae.1029-1037engCurcuminHigh-performance liquid chromatographyLiquid crystalsPerformance testsValidationArtigo10.1016/j.arabjc.2016.01.014Acesso restrito2-s2.0-8495821423391297805367242561427125996716282