Freitas, Helayne SoaresD'Agord Schaan, BeatrizSeraphim, Patricia MonteiroNunes, Maria TerezaMachado, Ubiratan Fabres2014-05-272014-05-272005-06-15Molecular and Cellular Endocrinology, v. 237, n. 1-2, p. 49-57, 2005.0303-7207http://hdl.handle.net/11449/68285Increased GLUT2 gene expression in the renal proximal tubule of diabetic rats is an adaptive condition, which may be important in the diabetic nephropathy development. We investigated the effects of insulin treatment upon the renal GLUT2 overexpression of diabetic rats. Acute treatment, surprisingly, induced a rapid further increase in GLUT2 mRNA content. Twelve hours after insulin injection, GLUT2 mRNA was twice the value of saline-injected rats (P < 0.001), when GLUT2 protein remained unchanged. In response to short-term treatment, both GLUT2 mRNA and protein were increased in 1-day treated rats (P < 0.05 versus saline-injected), decreasing after that, and reaching, within 6 days, values close to those of non-diabetic rats. Concluding, insulin treatment induced: initially, an additional upregulation of GLUT2 gene expression, involving posttranscriptional modulation; thereafter, downregulation of GLUT2 expression, which returns to non-diabetic levels. The former may be related to increased insulin concentration, the latter may be due to glycemic control. © 2005 Elsevier B.V. All rights reserved.49-57engAlloxanDiabetesGlucose transportersGLUT2 mRNAGLUT2 protein (rat kidney)Insulin treatmentNephropathyglucose transporter 2insulinmessenger RNAsodium chlorideanimal experimentanimal modelconcentration responsecontrolled studydiabetes mellitusdrug effectgene expression regulationgene overexpressiongenetic transcriptionglucose blood levelglucosuriainsulin treatmentkidney cortexmalenonhumanNorthern blottingpriority journalratupregulationWestern blottingAdaptation, PhysiologicalAnimalsBlood GlucoseDiabetes Mellitus, ExperimentalGene ExpressionGlucose Transporter Type 2Hypoglycemic AgentsInsulinKidney CortexMaleMonosaccharide Transport ProteinsRatsRats, WistarReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTime FactorsAcute and short-term insulin-induced molecular adaptations of GLUT2 gene expression in the renal cortex of diabetic ratsArtigo10.1016/j.mce.2005.03.005Acesso restrito2-s2.0-1954437937304110085990708710000-0003-2145-6640