Bueno, Paulo Roberto [UNESP]Gonçalves, Luís M.dos Santos, Fernanda C. [UNESP]dos Santos, Marcio L. [UNESP]Barros, Aquiles A.Faria, Ronaldo C.2014-05-272014-05-272012-12-01Analytical Letters, v. 46, n. 2, p. 258-265, 2012.0003-27191532-236Xhttp://hdl.handle.net/11449/73799Electrogravimetric analysis was performed on the consumption of the neurotransmitter Acetylcholine (ACh) by Acetylcholinesterase (AChE) in situ and in real time. Michaelis-Menten assumption was achieved by using an enzyme micro-reactor in which the total enzyme was anchored in a quartz crystal microbalance chip (QCM-chip) with a strategically engineered self-assembled monolayer (SAM) of alkanethiols, which can prevent diffusion-controlled or spatially restricted kinetics. The real-time frequency changes indicated the rate of the products formation from enzymatic reaction. The QCM-chip was tested showing that it could demonstrate AChE inhibition by physostigmine. © 2013 Copyright Taylor and Francis Group, LLC.258-265engAcetyl cholinesteraseAcetylcholineNeurotransmitterPhysostigmineQuartz-crystal microbalanceArtigo10.1080/00032719.2012.713065WOS:000312984400004Acesso restrito2-s2.0-8487185950404770459067332540000-0003-2827-0208