Andrade, Milena M.Barbosa, Aneli M.Bofinger, Matheus R.Dekker, Robert F. H.Messias, Josana M.Guedes, Carmen L. B.Zaminelli, TiagoDe Oliveira, Bruno H. [UNESP]De Lima, Valéria M. G. [UNESP]Dall'Antonia, Luiz H.2014-05-272014-05-272013-08-01Applied Biochemistry and Biotechnology, v. 170, n. 7, p. 1792-1806, 2013.0273-22891559-0291http://hdl.handle.net/11449/76115The effects of soybean and castorbean meals were evaluated separately, and in combinations at different ratios, as substrates for lipase production by Botryosphaeria ribis EC-01 in submerged fermentation using only distilled water. The addition of glycerol analytical grade (AG) and glycerol crude (CG) to soybean and castorbean meals separately and in combination, were also examined for lipase production. Glycerol-AG increased enzyme production, whereas glycerol-CG decreased it. A 24 factorial design was developed to determine the best concentrations of soybean meal, castorbean meal, glycerol-AG, and KH2PO4 to optimize lipase production by B. ribis EC-01. Soybean meal and glycerol-AG had a significant effect on lipase production, whereas castorbean meal did not. A second treatment (22 factorial design central composite) was developed, and optimal lipase production (4,820 U/g of dry solids content (ds)) was obtained when B. ribis EC-01 was grown on 0.5 % (w/v) soybean meal and 5.2 % (v/v) glycerol in distilled water, which was in agreement with the predicted value (4,892 U/g ds) calculated by the model. The unitary cost of lipase production determined under the optimized conditions developed ranged from US$0.42 to 0.44 based on nutrient costs. The fungal lipase was immobilized onto Celite and showed high thermal stability and was used for transesterification of soybean oil in methanol (1:3) resulting in 36 % of fatty acyl alkyl ester content. The apparent K m and V max were determined and were 1.86 mM and 14.29 μmol min -1 mg-1, respectively. © 2013 Springer Science+Business Media New York.1792-1806engAgro-industrial residuesCeliteGlycerolImmobilizationLipase productionResponse surface methodSubmerged fermentationAgro-industrial residueFermentationOptimizationRadioactive waste vitrificationSubstratesalkyl groupbiodieselesterglycerolinfusorial earthmethanolsoybean oiltriacylglycerol lipasewaterbeanbiofuel productionBotryosphaeria ribiscastorbeanenzyme immobilizationenzyme kineticsenzyme synthesisfactorial designfungusmealnonhumannucleotide sequencenutrientpredictive valueresponse surface methodsoybeansubmerged fermentationGlycine maxRicinus communisArtigo10.1007/s12010-013-0309-9WOS:000322542900020Acesso restrito2-s2.0-84881233696