Lovaglio, R. B. [UNESP]Costa, S. G. V. A. O. [UNESP]Lima, C. J. B. [UNESP]Cortezi, M. [UNESP]Contiero, Jonas [UNESP]2013-09-302014-05-202013-09-302014-05-202010-08-01Research Journal of Biotechnology. Indore: Research Journal Biotechnology, v. 5, n. 3, p. 19-24, 2010.0973-6263http://hdl.handle.net/11449/20134Waste utilization for biosurfactant production is an important approach contributing to the reduction of waste treatment costs, while increasing the economic value of residues and reducing biosurfactant production costs: In an attempt to optimize rhamnolipid production by Pseudomonas aeruginosa LBI, the effects of the oil refinery waste as carbon source, C/N ratio, agitation speed, aeration rate, pH control and fed-batch culture conditions on the production of this biosurfactant were assessed in a bioreactor. Maximum production (16.9 g L(-1)) was attained at a C/N ratio of 23; agitation at 800 rpm and aeration at 2.0 vvm. The rhamnolipid solution (0.1%, w/v) exhibited surface and interface tensions of 25 mN/m and 0.63 mN/m respectively. The critical micelle concentration (CMC) was 73.9 mg L(-1). The optimal parameters used in this study promoted the formation of a useful biosurfactant with potential for many industrial applications.19-24engRhamnolipidsC/N ratioSoapstockAeration rate and Agitation speedArtigoWOS:000281391200005Acesso restrito9859154979447005