Silva, Cassiano C.Dekker, Robert F. H.Silva, Rui Sergio S. F.da Silva, Maria de Lourdes CorradiBarbosa, Aneli M.2014-05-202014-05-202007-08-01Process Biochemistry. Oxford: Elsevier B.V., v. 42, n. 8, p. 1254-1258, 2007.1359-5113http://hdl.handle.net/11449/6797The addition of soybean oil and Tween 80 was evaluated with the objective of increasing the production of botryosphaeran, an exopolysaccharide (EPS) of the (1 -> 3 ;1 -> 6)-beta-D-glucan type produced by the fungus Botowsphaeria rhodina MAMB-05. Factorial design and analysis by response surface methodology was developed to select the main factors that would affect and enhance EPS production. The optimized culture conditions were: 40g l(-1) glucose with 10ml l(-1) soybean oil, and 4.5 ml l(-1) Tween 80, during 72h cultivation at 28 degrees C (180 rpm) and initial pH 5.7. The predicted result for botryosphaeran production was 8.22 +/- 1.36 g l(-1), and compared with the experimental value of 7.74 +/- 0.13 g l(-1) . Partial characterization of the botryosphaeran produced under the optimized conditions showed one type of polysaccharide with P-glycosidic linkages containing glucose as monosaccharide. (c) 2007 Elsevier Ltd. All rights reserved.1254-1258engbotryosphaeranBotryosphaeria rhodina MAMB-05exopolysaccharidesoybean oiltween 80response surface methodologyArtigo10.1016/j.procbio.2007.05.009WOS:000248975400011Acesso restrito