Gonzales Silverio-Ruiz, KarinaTraverso Martinez, Aurora EsmeraldaPompermaier Garlet, GustavoFregonesi Barbosa, CarolinaSantana Silva, JoaoBarreto Cicarelli, Regina MariaValentini, Sandro Roberto [UNESP]Georges Abi-Rached, Ricardo SarnihRossa, Carlos Junior2014-05-202014-05-202007-08-01Cytokine. London: Academic Press Ltd Elsevier B.V. Ltd, v. 39, n. 2, p. 130-137, 2007.1043-4666http://hdl.handle.net/11449/31237This study evaluated the effects of bFGF and TGF-beta, individually and combined, on cell proliferation and collagen metabolism. Primary human periodontal ligament cells were stimulated with two concentrations (I and 10 ng/ml) of each growth factor, both individually and combined. Proliferation was determined by a commercial biochemical assay. Real time RT-PCR determined gene expression of NMP-1 and -2, collagen types I and III, TIMP-1, -2 and -3. Autocrine effects on synthesis of bFGF and TGF-beta were evaluated by ELISA. Only TGF-beta, either isolated or associated with bFGF, significantly increased cell proliferation. TGF-beta had anabolic effects, increasing expression of type I and III collagen as well as of TIMPs, whereas bFGF had opposite effects. When bFGF and TGF-beta were associated, the anabolic effects prevailed. Synthesis of TGF-beta was induced only by the association of lower concentrations of the growth factors, whereas there was a dose-dependent production of bFGF. It is concluded that bFGF had a predominantly catabolic effect, and TGF-beta exerted an anabolic effect on hPDL cells. (c) 2007 Elsevier Ltd. All rights reserved.130-137enghuman periodontal ligament cellstransforming growth factor betabasic fibroblast growth factorcollagenmatrix metalloproteasestissue inhibitor of matrix metalloproteasesperiodontal regenerationArtigo10.1016/j.cyto.2007.06.009WOS:000250913700006Acesso restrito5333250355049814