Ullah, Anwar [UNESP]de Giuseppe, Priscila OliveiraMurakami, Mario TyagoTrevisan-Silva, DilzaMartins Wille, Ana CarolinaChaves-Moreira, DanieleGremski, Luiza Helenada Silveira, Rafael BertoniSennf-Ribeiro, AndreaChaim, Olga MeiriVeiga, Silvio SanchesArni, Raghuvir Krishnaswamy [UNESP]2014-05-202014-05-202011-02-01Acta Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell, v. 67, p. 234-236, 2011.1744-3091http://hdl.handle.net/11449/22098Phospholipases D are the major dermonecrotic component of Loxosceles venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses. Phospholipases D can be classified into two classes depending on their catalytic efficiency and the presence of an additional disulfide bridge. In this work, both wild-type and H12A-mutant forms of the class II phospholipase D from L. intermedia venom were crystallized. Wild-type and H12A-mutant crystals were grown under very similar conditions using PEG 200 as a precipitant and belonged to space group P12(1)1, with unit-cell parameters a = 50.1, b = 49.5, c = 56.5 angstrom, beta = 105.9 degrees. Wild-type and H12A-mutant crystals diffracted to maximum resolutions of 1.95 and 1.60 angstrom, respectively.234-236engArtigo10.1107/S1744309110050931WOS:000287030600013Acesso abertoWOS000287030600013.pdf91625089789458870000-0003-2460-1145