Macente, SaraLeite, Clarice Queico Fujimura [UNESP]Barreto Santos, Adolfo Carlos [UNESP]Dias Siqueira, Vera LuciaCosmo Machado, Luzia NeriMarcondes, Nadir RodriguesHirata, Mario HiroyukiCrespo Hirata, Rosario DominguezCardoso, Rosilene Fressatti2014-12-032014-12-032013-01-01Biomed Research International. New York: Hindawi Publishing Corporation, 6 p., 2013.2314-6133http://hdl.handle.net/11449/113446Current study evaluated the hsp65 Nested PCR Restriction Fragment Length Polymorphism Analysis (hsp65 Nested PCR-PRA) to detect and identify Mycobacterium tuberculosis complex directly in clinical samples for a rapid and specific diagnosis of tuberculosis (TB). hsp65 Nested PCR-PRA was applied directly to 218 clinical samples obtained from 127 patients suspected of TB or another mycobacterial infection from July 2009 to July 2010. The hsp65 Nested PCR-PRA showed 100% sensitivity and 95.0 and 93.1% specificity in comparison with culture and microscopy (acid fast bacillus smear), respectively. hsp65 Nested PCR-PRA was shown to be a fast and reliable assay for diagnosing TB, which may contribute towards a fast diagnosis that could help the selection of appropriate chemotherapeutic and early epidemiological management of the cases which are of paramount importance in a high TB burden country.6engArtigo10.1155/2013/391549WOS:000326519700001Acesso abertoWOS000326519700001.pdf2114570774349859