Different bacterial models for in vitro induction of non-cavitated enamel caries-like lesions: microhardness and polarized light miscroscopy analyses

De Campos, Priscila HernandezSanabe, Mariane EmiRodrigues, Jonas AlmeidaDuarte, Danilo AntonioBotti Rodrigues Santos, Maria TeresaGuare, Renata OliveiraDuque, Cristiane [UNESP]Lussi, AdrianDiniz, Michele Baffi2015-10-212015-10-212015-06-01Microscopy Research And Technique, v. 78, n. 6, p. 444-451, 2015.1059-910Xhttp://hdl.handle.net/11449/128509The aim of this study was to compare different bacterial models for in vitro induction of non-cavitated enamel caries-like lesions by microhardness and polarized light microscopy analyses. One hundred blocks of bovine enamel were randomly divided into four groups (n=25) according to the bacterial model for caries induction: (A) Streptococcus mutans, (B) S. mutans and Lactobacillus acidophilus, (C) S. mutans and L. casei, and (D) S. mutans, L. acidophilus, and L. casei. Within each group, the blocks were randomly divided into five subgroups according to the duration of the period of caries induction (4-20 days). The enamel blocks were immersed in cariogenic solution containing the microorganisms, which was changed every 48 h. Groups C and D presented lower surface hardness values (SMH) and higher area of hardness loss (S) after the cariogenic challenge than groups A and B (P<0.05). As regards lesion depth, under polarized light microscopy, group A presented significantly lower values, and groups C and D the highest values. Group B showed a higher value than group A (P<0.05). Groups A and B exhibited subsurface caries lesions after all treatment durations, while groups C and D presented erosion-type lesions with surface softening. The model using S. mutans, whether or not it was associated with L. acidophilus, was less aggressive and may be used for the induction of non-cavitated enamel caries-like lesions. The optimal period for inducing caries-like lesions was 8 days. Microsc. Res. Tech. 78:444-451, 2015. (c) 2015 Wiley Periodicals, Inc.444-451engTooth enamelTooth demineralizationBacteriaHardness testsMicroscopyDifferent bacterial models for in vitro induction of non-cavitated enamel caries-like lesions: microhardness and polarized light miscroscopy analysesArtigo10.1002/jemt.22493WOS:000355232000002Acesso restrito56518745094936170000-0002-2575-279X