Giese, Ellen C. [UNESP]Dekker, Robert F.H.Scarminio, Ieda S.Barbosa, Aneli M.da Silva, Roberto [UNESP]2014-05-272014-05-272011-01-15Biochemical Engineering Journal, v. 53, n. 2, p. 239-243, 2011.1369-703Xhttp://hdl.handle.net/11449/72299Botryosphaeria rhodina MAMB-05 produced β-1,3-glucanases and botryosphaeran when grown on glucose, while Trichoderma harzianum Rifai only produced the enzyme. A comparison of long-term cultivation (300h) by B. rhodina demonstrated a correlation between the formation of botryosphaeran (48h) and its consumption (after 108h), and de-repression of β-1,3-glucanase synthesis when glucose was depleted from the nutrient medium, whereas for T. harzianum enzyme production commenced during exponential growth. Growth profiles and levels of β-1,3-glucanases produced by both fungi on botryosphaeran also differed, as well as the production of β-1,3-glucanases and β-1,6-glucanases on glucose, lactose, laminarin, botryosphaeran, lasiodiplodan, curdlan, Brewer's yeast powder and lyophilized fungal mycelium, which were dependent upon the carbon source used. A statistical mixture-design used to optimize β-1,3-glucanase production by both fungi evaluated botryosphaeran, glucose and lactose concentrations as variables. For B. rhodina, glucose and lactose promoted enzyme production at the same levels (2.30UmL -1), whereas botryosphaeran added to these substrates exerted a synergic effect favorable for β-glucanase production by T. harzianum (4.25UmL -1). © 2010 Elsevier B.V.239-243engβ-1,3-GlucanasesBotryosphaeranStatistical mixture-design optimizationBotryosphaeria rhodina MAMB-05Carbon sourceCurdlanDe-repressionEnzyme productionExponential growthGlucanaseLaminarinsNutrient mediumSynergic effectsTrichoderma harzianum RifaiDesignEnzymesFungiGlucoseMixturesOptimization1,3 beta glucanasecurdlanglucoselactoselaminaranbotryosphaeria rhodinacomparative studycorrelation analysisenzyme substrateenzyme synthesisfungusHypocrea lixiimyceliumnonhumanpriority journalstatistical analysisBotryosphaeria rhodinaComparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-designArtigo10.1016/j.bej.2010.10.013Acesso aberto2-s2.0-786499601622-s2.0-78649960162.pdf9424175688206545