Marques, Isabella da Silva VieiraCruz, Nilson Cristino da [UNESP]Landers, RichardYuan, Judy Chia-ChunMesquita, Marcelo FerrazSukotjo, CortinoMathew, Mathew T.Barão, Valentim Adelino Ricardo2015-12-072015-12-072015Biointerphases, v. 10, n. 4, p. 1-11, 2015.1559-4106http://hdl.handle.net/11449/131447The objectives of the present study were to produce bioactive coatings in solutions containing Ca, P, and Si by plasma electrolytic oxidation (PEO) on commercially pure titanium, to investigate the influence of different electrolytes concentration and treatment duration on the produced anodic films and to evaluate biocompatibility properties. The anodic films were characterized using scanning electron microscopy, energy-dispersive spectroscopy, atomic force microscopy, and x-ray diffraction and x-ray photoelectron spectroscopies. The surface energy and roughness were also evaluated. PEO process parameters influenced the crystalline structure formation and surface topography of the anodic films. Higher Ca content produced larger porous (volcanolike appearance) and thicker oxide layers when compared to the lower content. Treatment duration did not produce any topography difference. The treatment modified the surface chemistry, producing an enriched oxide layer with bioactive elements in the form of phosphate compounds, which may be responsible for mimicking bone surface. In addition, a rough surface with increased surface energy was generated. Optimal spreading and proliferation of human mesenchymal stem cells was achieved by PEO treatment, demonstrating excellent biocompatibility of the surface. The main finding is that the biofunctionalization with higher Ca/P on Ti-surface can improve surface features, potentially considered as a candidate for dental implants.1-11engArtigo10.1116/1.4932579Acesso restrito715732722004813826446191