Hossepian de Lima, V. F MMoreira-Filho, C. A.De Bem, A. R.Jorge, W. [UNESP]2014-05-272014-05-271993-06-01Theriogenology, v. 39, n. 6, p. 1343-1352, 1993.0093-691Xhttp://hdl.handle.net/11449/64377Murine and bovine embryos were sexed by using polyclonal and monoclonal anti-H-Y antibodies in cytotoxic and immunofluorescence assays. The specificity of 3 different sources of antibodies are compared. Sexing was accomplished by complement-mediated cytotoxicity or by H-Y antigen detection using a anti-mouse Ig fluorescein conjugate. Cytogenetic ascertainment for correct sex identification yielded a score of about 85% in both species, while the immunofluorescence assay yielded a score of 83%. © 1993.1343-1352enganti-H-Y antibodiesembryoimmunological assayssexingArtigo10.1016/0093-691X(93)90236-XWOS:A1993LF25800013Acesso restrito2-s2.0-38249001637