Prudencio, Carlos RobertoGomes da Costa, Vivaldo [UNESP]Rocha, Leticia Barbozada Costa, Hernan Hermes MonteiroOrts, Diego José Belatoda Silva Santos, Felipe RochaRahal, Paula [UNESP]Lino, Nikolas Alexander Borsato [UNESP]da Conceição, Pâmela Jóyce Previdelli [UNESP]Bittar, Cintia [UNESP]Machado, Rafael Rahal GuaragnaDurigon, Edison LuizAraujo, João Pessoa [UNESP]Polatto, Juliana Moutinhoda Silva, Miriam Aparecidade Oliveira, Joyce AraújoMitsunari, ThaisPereira, Lennon RamosAndreata-Santos, Robertde Souza Ferreira, Luís CarlosLuz, DanielaPiazza, Roxane Maria Fontes2023-07-292023-07-292023-03-01Viruses, v. 15, n. 3, 2023.1999-4915http://hdl.handle.net/11449/249851Zika virus (ZIKV), a mosquito-borne pathogen, is an emerging arbovirus associated with sporadic symptomatic cases of great medical concern, particularly among pregnant women and newborns affected with neurological disorders. Serological diagnosis of ZIKV infection is still an unmet challenge due to the co-circulation of the dengue virus, which shares extensive sequence conservation of structural proteins leading to the generation of cross-reactive antibodies. In this study, we aimed to obtain tools for the development of improved serological tests for the detection of ZIKV infection. Polyclonal sera (pAb) and a monoclonal antibody (mAb 2F2) against a recombinant form of the ZIKV nonstructural protein 1 (NS1) allowed the identification of linear peptide epitopes of the NS1 protein. Based on these findings, six chemically synthesized peptides were tested both in dot blot and ELISA assays using convalescent sera collected from ZIKV-infected patients. Two of these peptides specifically detected the presence of ZIKV antibodies and proved to be candidates for the detection of ZIKV-infected subjects. The availability of these tools opens perspectives for the development of NS1-based serological tests with enhanced sensitivity regarding other flaviviruses.engdiagnosisepitopesimmunoassaysmonoclonal antibodyNS1 proteinpeptidesZika virusArtigo10.3390/v150306542-s2.0-85152253473