Use of reverse transcriptase polymerase chain reaction (RT-PCR) in molecular screening of Newcastle disease virus in poultry and free-living bird populations

de Oliveira Torres Carrasco, AdrianoRodrigues, Juliana Nogueira MartinsSeki, Meire Christina [UNESP]de Moraes, Fabricio Edgar [UNESP]Silva, Jaqueline Raymondi [UNESP]Durigon, Edison LuisPinto, Aramis Augusto [UNESP]2014-05-272014-05-272012-09-17Tropical Animal Health and Production, v. 45, n. 1, p. 569-576, 2012.0049-47471573-7438http://hdl.handle.net/11449/73577The aim of this study was to evaluate a simple molecular method of reverse transcriptase polymerase chain reaction (RT-PCR) to differentiate Newcastle disease virus strains according to their pathogenicity, in order to use it in molecular screening of Newcastle disease virus in poultry and free-living bird populations. Specific primers were developed to differentiate LaSota-LS-(vaccine strain) and Sao Joao do Meriti-SJM-strain (highly pathogenic strain). Chickens and pigeons were experimentally vaccinated/infected for an in vivo study to determine virus shedding in feces. Validation of sensitivity and specificity of the primers (SJM and LS) by experimental models used in the present study and results obtained in the molecular analysis of the primers by BLAST made it possible to generalize results. The development of primers that differentiate the level of pathogenicity of NDV stains is very important, mainly in countries where real-time RT-PCR is still not used as a routine test. These primers were able to determine the presence of the agent and to differentiate it according to its pathogenicity. © 2012 Springer Science+Business Media B.V.569-576engDiagnosisExperimental infectionNewcastle disease virusPoultryAvesColumbaGallus gallusprimer DNAanimalanimal diseasebird diseasechickenevaluationfecesgeneticsisolation and purificationmethodologyNewcastle diseaseNewcastle disease paramyxoviruspigeons and dovesreverse transcription polymerase chain reactionsensitivity and specificityvirologyvirus sheddingAnimalsChickensColumbidaeDNA PrimersFecesNewcastle DiseasePoultry DiseasesReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityVirus SheddingUse of reverse transcriptase polymerase chain reaction (RT-PCR) in molecular screening of Newcastle disease virus in poultry and free-living bird populationsArtigo10.1007/s11250-012-0261-7WOS:000314046100031Acesso restrito2-s2.0-84872827481