Braga, Leticia da Conceicao [UNESP]Silva, Luciana MariaAlvares da Silva Ramos, Ana PaulaPiedade, Josiane BarbosaTeixeira Vidigal, Paula VieiraTraiman, Paulo [UNESP]Silva Filho, Agnaldo Lopes da [UNESP]2014-12-032014-12-032014-02-01Biomedicine & Pharmacotherapy. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 68, n. 1, p. 87-91, 2014.0753-3322http://hdl.handle.net/11449/112245Despite impressive research efforts, the biology of epithelial ovarian cancer (EOC) remains poorly understood and alterations in the expression of CASPASE-8 contribute to a worse tumor prognosis. This study assesses the methylation of the CpG island within the CASPASE-8 promoter and CASPASE-8 gene expression both in cystadenoma tumors and in primary and metastatic EOC. DNA and RNA were obtained from women with normal ovarian tissues (n = 18), ovarian serous cystadenoma tumors (n = 11) and EOC (n = 16) using Trizol (R). The methylation frequency of the CpG island in the CASPASE-8 promoter was assessed using the methylation-specific PCR assay after DNA bisulfite conversion. Quantitative PCR was performed to quantify the relative levels of CASPASE-8 in each sample. The differences between samples with each group were evaluated using the Mann-Whitney U and Kruskal-Wallis tests as indicated. Hemimethylation of the CASPASE-8 promoter was found in 11.8% of the normal ovary samples, 20% of the cystadenoma tumors and 20% of the metastatic EOC, while methylation of the CASPASE-8 promoter was absent in the EOC primary tissues (P = 0.047). An increased CASPASE-8 expression level was observed in all tumor groups. Significant differences were observed in the CASPASE-8 expression levels when compared with all ovarian tumor groups (P = 0.0278). Promoter DNA methylation did not associate with expression levels of CASPASE-8, suggesting the presence of other mechanisms in relation to gene expression control in EOC; thus providing a better understanding of this complex disease. (C) 2013 Elsevier Masson SAS. All rights reserved.87-91engEpithelial ovarian cancerCASPASE-8ExpressionArtigo10.1016/j.biopha.2013.12.004WOS:000332448400014Acesso restrito8334785337106990