Soares, Felipe Augustodos Santos, Bianca MartinsRosa, Stefany LaryssaLoiola, Saulo Hudson NeryStéfano, Vitória CastilhoProença, Letícia RodriguesSuzuki, Celso Tetsuo NagaseSabadini, EdvaldoBresciani, Katia Denise Saraiva [UNESP]Falcão, Alexandre XavierGomes, Jancarlo Ferreira2023-07-292023-07-292022-12-01Tropical Medicine and International Health, v. 27, n. 12, p. 1044-1052, 2022.1365-31561360-2276http://hdl.handle.net/11449/248999Objective: Ova and parasite (O&P) examination is recommended for the laboratory diagnosis of agents causing parasitic infections; however, this exam requires scientific and technological improvements to enhance its diagnostic validity. Dissolved air flotation (DAF) is an efficient technical principle separating suspended solids in a liquid medium. We aimed to develop and validate a new procedure for intestinal parasite detection with DAF. Methods: In this study, we collected samples from 500 volunteers, screened them by direct examination, and transferred the material to tubes using the Three Faecal Test (TF-Test) for triplicate DAF tests. We evaluated physical–chemical parameters and DAF prototype components through quantifying parasites recovered from floated and non-floated regions of the flotation column. The DAF operation protocol was validated with the gold standard results. Results: The 10% saturated volume proportion and cationic surfactant showed regularity and high parasite recovery (80%). Modifications of the needle device did not influence parasite recovery (p > 0.05). Sensitivity, specificity, accuracy and kappa agreement obtained with the DAF protocol were 91%, 100%, 93% and substantial (k = 0.64), respectively. Conclusion: The DAF principle could be used to process faecal samples in routine laboratory exams, enabling intestinal parasite detection.1044-1052engdiagnostic techniques and proceduresflotationparasitic diseasesparasitologyArtigo10.1111/tmi.138272-s2.0-85141379174