Guimarães, M. C. M.Soares, Christiane Pienna [UNESP]Donadi, Eduardo AntônioDerchain, Sophie F. M.Andrade, Liliana Aparecida Lucci de AngeloSilva, Tarsia G. A. [UNESP]Hassumi, M. K.Simões, Renata T.Miranda, Fabiana A.Lira, Régia C. P.Crispim, Janaína OliveiraSoares, Edson Garcia2016-01-282016-01-282010The Journal of Histochemistry and Cytochemistry, v. 58, n. 5, p. 405-411, 2010.0022-1554http://hdl.handle.net/11449/133710Human leukocyte antigen-G (HLA-G) is a non-classical major histocompatibility complex class Ib molecule that acts as a specific immunosuppressor. Some studies have demonstrated that human papillomavirus (HPV) seems to be involved in lower or absent HLA-G expression, particularly in cervical cancer. In this study, we performed a cross-sectional study, systematically comparing the qualitative expression of the HLA-G5 isoform in invasive cervical carcinoma (ICC), stratifying patients according to the presence [ICC with metastasis (ICCW)] and absence [ICC without metastasis (ICCWT)] of metastasis, correlating these findings with interference of HPV and demographic and clinical variables. Seventy-nine patients with a diagnosis of ICC were stratified into two groups: ICCWT (n=52 patients) and ICCW (n=27). Two biopsies were collected from each patient (one from the tumor lesion and one from a lymph node). Immunohistochemistry analyses were performed for the HLA-G5 isoform, for HPV detection, and virus typing. HLA-G5 isoform molecules were detected in 25 cases (31.6%), 17 (32.7%) without metastasis and 8 (29.6%) with metastasis. HPV was detected in the cervical lesions of 74 patients (93.7%), but low expression of the HLA-G5 isoform was observed in all HPV-related cases. These findings are important; however, additional studies are necessary to identify the influence of HPV with HLA-G5 isoform expression on invasive cervical malignancies.405-411engInvasive cervical carcinomaHLA-G5 isoformHPVImmunohistochemistryPCRArtigo10.1369/jhc.2009.954131Acesso restrito311280309422820715774797705609313285282420989060231507305342374717680252903736699338770260143276238252133074659887787133821357710000-0003-1740-7360