Nunes, Erika Takagi [UNESP]Camargo Mathias, Maria Izabel [UNESP]Bechara, Gervásio Henrique [UNESP]2014-05-272014-05-272006-10-01Experimental Parasitology, v. 114, n. 2, p. 109-117, 2006.0014-48941090-2449http://hdl.handle.net/11449/69123This study investigates the presence and the localization of acid phosphatase and ATPase in the salivary glands of Rhipicephalus (Boophilus) microplus female ticks during feeding. Semi-engorged females showed a larger amount of acid phosphatase compared to those at beginning of feeding, localized mainly in the apical portion of the secretory cells, and in the basal labyrinth of the interstitial cells. Ultrastructural observations also demonstrated its presence in secretion granules and inside some nuclei of secretory cells at beginning of feeding. Acid phosphatase in a free form probably has a hemolymph and/or ribosomal origin and participates in salivary gland secretion control. ATPase was detected in basal membrane of all types of acini and/or in the cytoplasm of the secretory cells at both feeding stages. The enzyme activities found strongly suggests that cell death by apoptosis occurs during the degenerative process. © 2006 Elsevier Inc. All rights reserved.109-117engAcid phosphataseATPaseCattle-tickCytochemistryHistochemistryRhipicephalus (Boophilus) microplusSalivary glandsacid phosphataseadenosine triphosphataseanimal cellanimal tissueapoptosisbasement membraneBoophilus micropluscontrolled studycytoplasmenzyme activityenzyme localizationfeedingfemalehemolymphnonhumanpriority journalsalivary glandsalivationsecretory cellsecretory granuleAcid PhosphataseAdenosine TriphosphatasesAnimalsCattleCell NucleusFeeding BehaviorFemaleHistocytochemistryMicroscopy, Electron, TransmissionRhipicephalusSalivary GlandsSecretory VesiclesAcariBoophilusIxodidaIxodidaeArtigo10.1016/j.exppara.2006.02.022WOS:000241172300007Acesso restrito2-s2.0-33748691666