Mol, Juliana P. S.Guedes, Andressa C. B.Eckstein, CamilaQuintal, Amanda P. N.Souza, Tayse D.Mathias, Luis A. [UNESP]Haddad, Joao Paulo A.Paixao, Tatiane A.Santos, Renato L.2020-12-102020-12-102019-11-21Journal Of Veterinary Diagnostic Investigation. Thousand Oaks: Sage Publications Inc, v. 32, n. 1, p. 77-86, 2020.1040-6387http://hdl.handle.net/11449/194997Canine brucellosis is an infectious and contagious disease associated with reproductive losses in breeding kennels. As a zoonotic disease, it poses a risk to human health, especially for veterinarians and breeders who handle materials potentially contaminated with Brucella canis. However, canine brucellosis is a neglected and underestimated disease given the difficulties in establishing a definitive diagnosis. We evaluated the frequency of detection of B. canis in 5 breeding kennels by using various serologic methods and PCR. Circulation of B. canis in these kennels was confirmed by bacterial isolation. The frequency of positive serologic results varied from 6.3% by AGID to 16.5% by dot-ELISA. There was no positive serology for smooth Brucella. PCR testing was positive in 13.9% of samples. The only detection tests with reasonable agreement were PCR and 2ME-MAT. The diagnosis of canine brucellosis remains challenging. The use of a single laboratory method, or even the use of different laboratory methods, may not be sufficient to reach a definitive diagnosis.77-86engbreeding kennelsBrucella canisdetection methodsreproductive diseaseszoonosisArtigo10.1177/1040638719891083WOS:000498126400001