Giacomelli, LRBHelbel, C.Ogassawara, RLNBarreto, AMWMartins, F. M.Cardoso, C. L.Leite, CQF2014-05-202014-05-202005-08-01Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 8, p. 4245-4248, 2005.0095-1137http://hdl.handle.net/11449/7530Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. The smears were decontaminated by the tube and slide techniques. Phenol at 5%, glutaraldehyde at 2%, and buffered formalin at 3.7% for 1 min (tube technique) or for 10 min (slide technique) were effective in decontaminating sputum smears and preserved cell morphology and quantitative acid-fast microscopy results.4245-4248engArtigo10.1128/JCM.43.8.4245-4248.2005WOS:000231136800107Acesso abertoWOS000231136800107.pdf