Soares, AMVCCalvi, Sueli Aparecida [UNESP]Peracoli, MTSFernandez, A. C.Dias-Melicio, Luciane Alarcão [UNESP]Dos Anjos, A. R.2014-05-202014-05-202001-04-01Immunology. Oxford: Blackwell Science Ltd, v. 102, n. 4, p. 480-485, 2001.0019-2805http://hdl.handle.net/11449/11756The effect of indomethacin (Indo), a cyclo-oxygenase inhibitor, on the monocyte-mediated killing of a low-(Pb265) and a high-(Pb18) virulence strain of Paracoccidioides brasiliensis was examined. The Pb18 strain was not killed by either non-activated or interferon-gamma (IFN-gamma)-activated human monocytes but these cells did show fungicidal activity if pretreated with Indo. In contrast with IFN-gamma tumour necrosis factor-alpha (TNF-alpha) was very effective at stimulating the fungicidal activity of monocytes. While the low-virulence strain, Pb265, could not be killed by monocytes, cells preincubated with IFN-gamma demonstrated fungicidal activity. The killing of this strain was also induced by pretreatment of monocytes with Indo. The results suggest a negative role for prostaglandins, which are synthesized via the cyclo-oxygenase pathway, in the regulation of monocyte-mediated killing of virulent and avirulent strains of P. brasiliensis and that TNF-alpha generation during the fungus-monocyte interaction is more important in the killing of Pb265 than Pb18.480-485engArtigo10.1046/j.1365-2567.2001.01179.xWOS:000168378200012Acesso abertoWOS000168378200012.pdf2179450022699059